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Perfomance of Pooling

Example:
100000 samples with prevalence of 2%
2000 positive / 98000 negative

100000

One-By-One

The common One-By-One testing method creates bottlenecks on the basis of testing each sample on its own.

testing 100.000 samples,
one-by-one
will require 100.000 tests.

27525

One-time Pooling

Labs have the capability to pool together multiple samples into a singe test tube, and that might provide a solution to save resources like reagent and time.

testing 100.000 samples,
one-time-pooling of 8
will require 27.525 tests.

saves ~70% of resources

20778

Repeated Pooling

Analysed and resolved pools will be tested again in smaller pools to reduce resource requirements like reagent and time.

testing 100.000 samples,
repeated-pooling of 16 / 4
will require 20.778 tests.

saves ~80% of resources

Source Samples

All source samples are available and collected on a 96 SBS rack for the laboratory process.

First Pooling ( 16 )

16 samples are pooled together in a single tube to get a test result of the sample group.

pool 1,2,5 = positive
pool 3,4,6 = negative

All samples of the negative pools have a negative result

Second Pooling ( 4 )

Each positive pool will be splitted in new groups of 4 samples. So we have a new list of pools.

pool 1,7,11 = positive
remaining = negative

All samples of the negative pools have a negative result

Final Analysis

Each source sample of the positive pools from the second pooling will be tested.

A1, A2, B1 = negative
B2 = positive
E3, E4, F3 = negative
F4 = positive
E8, F8, F9 = negative
E9 = positive

Summary

First Pooling: 6 tests ( 3 positive pools, 48 negative samples )
Second Pooling: 12 tests ( 3 positive pools, 36 negative samples )
Final Analysis: 12 tests ( 3 positive samples, 9 negative samples )

Hardware / Software

We have decades of experience in automated pooling solutions
for high througput laboratory analysis systems.

1

sample scanning

capturing of sample id, date, time and position on the rack for further preparation and liquidhandling

2

liquidhandling / pooling / isolation

robot assysted sample preparation, pooling and isolation/cherry picking of samples after the PCR analysis.

3

realtime PCR

results of the PCR will be stored to each sample in the database for automatic cherry picking and further pooling

4

Database

every state of each sample could be tracked with the installed database solution

Perfomance of Pooling

Example:
100000 samples with prevalence of 2%
2000 positive / 98000 negative

100000

One-By-One

27525

One-time Pooling

20778

Repeated Pooling

Pooling explained

example repeated pooling process with an 16 / 4 / 1 method

Source Samples

First Pooling ( 16 )

Second Pooling ( 4 )

Final Analysis

Summary

First Pooling: 6 tests ( 3 positive pools, 48 negative samples )
Second Pooling: 12 tests ( 3 positive pools, 36 negative samples )
Final Analysis: 12 tests ( 3 positive samples, 9 negative samples )

Hardware / Software

We have decades of experience in automated pooling solutions
for high througput laboratory analysis systems.

1

sample scanning

2

liquidhandling / pooling / isolation

3

realtime PCR

4

Database

Perfomance of Pooling

Example:100000 samples with prevalence of 2%2000 positive / 98000 negative

100000

One-By-One

27525

One-time Pooling

20778

Repeated Pooling

Pooling explained

example repeated pooling process with an 16 / 4 / 1 method

Source Samples

First Pooling ( 16 )

Second Pooling ( 4 )

Final Analysis

Summary

First Pooling: 6 tests ( 3 positive pools, 48 negative samples ) Second Pooling: 12 tests ( 3 positive pools, 36 negative samples ) Final Analysis: 12 tests ( 3 positive samples, 9 negative samples )

Hardware / Software

We have decades of experience in automated pooling solutions for high througput laboratory analysis systems.

1

sample scanning

2

liquidhandling / pooling / isolation

3

realtime PCR

4

Database

Example:
100000 samples with prevalence of 2%
2000 positive / 98000 negative

First Pooling: 6 tests ( 3 positive pools, 48 negative samples )
Second Pooling: 12 tests ( 3 positive pools, 36 negative samples )
Final Analysis: 12 tests ( 3 positive samples, 9 negative samples )

We have decades of experience in automated pooling solutions
for high througput laboratory analysis systems.